PhD student and a Post doc
Sanquin Research is looking for a PhD student and a Post doc – Interactions of hemostatic proteins with the vasculature; go with the flow?
Hemostasis is achieved by a delicate triadic interplay between the vessel wall, blood platelets and coagulation factors. Blood flow is an integral part of hemostasis, yet is completely overlooked in most studies on hemostatic protein interactions with the vasculature. The current project aims to identify the effect of blood flow on crosstalk between hemostatic proteins and the vasculature. More specifically, this project aims to 1) dissect flow-dependent receptor-ligand interactions 2) identify flow-induced conformational changes in membrane receptors and the vascular membrane surface and 3) uncover the role of key players of primary hemostasis, including platelets and VWF, and secondary hemostasis, including FVIII, in coagulation-induced signaling in the presence and absence of flow. For these studies, advanced mass spectrometry-based approaches are used including chemical footprinting using tandem-mass tags, hydrogen-deuterium exchange, cell surface proteomics and phosphoproteomics. Mass spectrometry-based studies will be complemented using biochemical and cell biological approaches including surface plesmon resonance and confocal microscopy. These studies will help to understand mechanosensory protein-receptor interactions and coagulation-induced vascular signaling. Understanding these interactions and signaling events is essential to develop rational approaches for the design of coagulation factors with an improved function and to identify potential novel targets for therapeutic intervention of hemostatic disorders.
You will work in a multidisciplinary international team that focuses on the biology of blood platelets coagulation proteins and cells of the haemostatic system. This team is embedded in the Department of Plasma Proteins, where 25-30 fellow colleagues (post-docs, PhD students and technicians) are studying various aspects of bleeding disorders. The department’s research program benefits from the excellent research facilities at Sanquin, which include the newest technology for molecular cell biology, protein engineering and mass spectrometry.
Where you will work as a PhD Student / Post Doc:
Sanquin Research performs basic, applied, and clinically oriented research in the field of blood and blood transfusion. Our research is focussed on haematology, blood coagulation, immunology, cellular therapies and donor studies. Sanquin offers an international, competitive, multidisciplinary research environment with state-of-the-art facilities. With over 200 colleagues, our focus is both on fundamental and applied research. We are interested in meeting ambitious researchers with training in life sciences, cell biology, biochemistry, or health sciences. Sanquin Blood Supply Foundation is responsible for the blood supply in the Netherlands, and is affiliated to the University of Amsterdam and other universities in the Netherlands.
We are looking for:
We are specifically interested in candidates who hold a PhD in Analytical Chemistry, Cell Biology, Molecular Sciences, Biochemistry or a related field. Candidates must have strong intellectual and experimental skills, in addition to experience/interest in mass spectrometry, bioinformatics, biochemistry and molecular biology.
- challenging project within a multi-disciplinary and enthusiastic team;
- salary and conditions are conform CAO Sanquin;
- working hours are fixed by mutual agreement;
- a temporary appointment for a period of 3 years (post-doc) or 4 years (PhD student);
- a 36-hour week;
- 8,33% year-end;
- by full-time employment 201 vacation hours
- reimbursement of travel expenses.
Interesse in de functie van PhD Student / Post Doc bij Sanquin in Amsterdam?
Reageer voor 16 april a.s. met je CV en motivatie via de bovenstaande "solliciteer direct" button.
For further information, please contact Dr. Maartje van den Biggelaar (tel. +31-20-5121289).
Canvassing in response to this advertisement is not welcome.
Sanquin does not accept unsolicited assistance from search or recruiting firms for this employment opportunity. All resumes (in any form) sent by search or recruiting firms to any employee at Sanquin without a valid written search agreement by HR Sanquin will be deemed to be public information. No fee will be paid in the event the candidate is hired by Sanquin as a result of sending Sanquin resumes without being asked or through any other means.