Research Technician, Adaptive Immunity Lab, Dept of Hematopoiesis, Amsterdam, 36h
MK 36-14 EXTEW
About the position:
We are now looking for a highly motivated research technician with a keen interest in cellular immunology. The candidate will support ongoing research projects in the Adaptive Immunity lab on the role of T cells in allo-immunisation and hematopoiesis. These projects involve many different cellular techniques, including isolation, analysis and culture of primary hematopoietic stem cells, as well as the isolation, analysis and stimulation of primary T cells from lymphoid tissue (mouse) or peripheral blood (human). The candidate will make use of various different techniques, such as multi-color flow cytometry, cell sorting, colony-assays, T cell stimulation assays, ELISAs, viral transduction (shRNA) and quantitative PCR. The candidate will work closely with other scientists in the team.
About the department:
Sanquin Research performs basic, applied and clinical research in the field of blood and blood transfusion. At the Adaptive Immunity Laboratory, which is part of the Department of Hematopoiesis, we investigate the mechanism by which the immune system generates effector T cells and antibody responses that are required for defense against invading pathogens, but that may also develop during allo-immunisation. We also examine the influence of these effector T cells on the formation of new blood cells in the bone marrow, with the aim to identify the cellular and molecular mechanisms by which the immune system regulates blood cell formation during infections. For these projects we collaborate closely with the Department of Experimental Immunohematology at Sanquin and the Department of Hematology from the AMC. Knowledge obtained from these research lines will provide insight into the consequences of (chronic) inflammation, such as the development of anemia. Moreover, this information can also be applied for the development of new therapies and the improvement of the cell transfusion products that Sanquin generates for clinical applications.
We are looking for:
- A highly motivated candidate, with a strong affinity for immunological research;
- Experience with flow cytometry, tissue culture and immunological techniques.
- Having obtained a certificate for conducting animal experiments (Article 9 or 12 according to Dutch law) is an advantage;
- A teamplayer that can also work independently within a team of technicians, PhD students and postdocs;
- Decent knowledge of the English language because of the international nature of our research group.
- a challenging position in a multidisciplinary and motivated research team;
- salary and conditions are conform CAO Sanquin;
- a 36-hour-week;
- reimbursement of travel expenses;
- a temporary appointment for a period of 3 years;
- working hours are fixed by mutual agreement;
- 8,33% year-end;
- by full-time employment 201 vacation hours.
For further information about this position, please contact Dr. Martijn A. Nolte, head of the Adaptive Immunity Laboratory at the department of Hematopoiesis on e-mail: firstname.lastname@example.org or visit our website www.hep.sanquin.nl.
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