Tissue-resident memory T cells (Trm) have been recently established as an important subset of memory cells that provide early and essential protection against re-infection. T cells with the phenotype of Trm have also been found to infiltrate tumor tissue, suggesting that these memory T cells may also have a protective role in cancer. The isolation, expansion and re-infusion of tumor-infiltrating T cells has been established as a successful therapy in cancer patients, but further improvements are still required to enhance protection. We aim to develop protocols that enhance tissue extraction, in vitro expansion and adoptive transfer of Trm to enable the use of Trm as a novel powerful tool for T cell therapy. The candidate will characterize the phenotype of Trm in healthy and tumor tissue of human primary material as well as in in vivo tumor models. The acquired information will be applied to optimize in vitro culture protocols of T cells to improve the yield of Trm in T cell cultures. The study of Trm in experimental infection and tumor models in addition to human primary material will enable the study of the in vivo efficacy of Trm in adoptive transfer settings.
Where you will work:
The Department of Hematopoiesis forms part of the Division Research of Sanquin. Sanquin is responsible for the supply of blood and blood products in The Netherlands. Sanquin Research is a large research institute with several departments studying blood cell development and function, plasma proteins and immunology (http://www.sanquin.nl/en/research). Sanquin provides a lively, internationally oriented, scientific environment with excellent facilities. The research is strongly multidisciplinary and includes molecular biology, flow cytometry, fluorescence microscopy, high throughput techniques (mass spectrometry, sequencing) and extensive cell culture of both adult and embryonic tissues.
We are looking for:
We invite highly motivated candidates with a strong affinity for immunological research with a HLO certificate or a Master’s degree in biology, biochemistry or any related discipline to apply for this position. A strong background in multi-color flow cytometry, cellular and molecular biology is required and experience with in vivo tumor and infection models is a clear advantage. We are looking for a flexible team player with good communication skills in English, who can work independently, but also as part of a multinational team.
- salary and working conditions conform CAO Sanquin;
- a temporary appointment for a period of 2 years;
- a fulltime contract of 36 hours per week;
- 8,33% end-of-the-year bonus;
- under full-time employment at least 201 hours of leave;
- reimbursement of travel expenses.
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